Real-time Polymerase Chain Reaction:Article Title: Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RNeasy RNA Extraction Mini Kit Qiagen Cat#74106 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit ThermoFisher Scientific Cat#A48310 AgPath-ID One-Step RT-PCR Reagents Life Technologies Cat#4387391 Deposited data Mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al. 2025 56 ) partner repository PXD064673 Cytoscape network NDEx (Pratt et al. 2015 57 ) https://doi.org/10.18119/N9SW4D Experimental models: Cell lines eHAP WT Horizon discovery N/A eHAP ANP32A/B/E-KO Barclay Lab (Sheppard et al. 2023 31 ) N/A .. A549-dox-Cas9 Martin-Sancho Lab (Martin-Sancho et al. 2021 58 ) N/A A549 WT This paper N/A A549 HMGB2-KO This paper N/A A549 RUVBL2-KO This paper N/A A549 RPAP2-KO This paper N/A 293T ATCC N/A MDCK ATCC N/A BCi-NS1.1 Ronald G. Crystal (Walters et al. 2013 59 ) N/A Oligonucleotides qPCR primers; see Table S4 This paper N/A vRNA, cRNA, mRNA, GAPDH RT-qPCR primers; see Table S4 This paper and Swann et al. 2023 11 N/A Primers for validation of CRISPR-Cas9 KO clones; see Table S4 This paper N/A siRNA pools, siNT, siANP32A, siANP32B; see Table S4 siGENOME, Dharmacon N/A sgRNA pools targeting HMGB2, RUVBL2, and RPAP2; see Table S4 Synthego N/A Negative control SpCas9 gRNA #1 Synthego N/A Recombinant DNA pCAGGS-PB1/PB2/PA/NP A/Puerto Rico/ 8/1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/England/195/ 2009 (pH1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/Victoria/3/ 75 (H3N2) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/turkey/ Turkey/05/2005 (H5N1) Barclay Lab N/A pCAGGS-PB1-SmBiT A/Puerto Rico/8/ 1934 (H1N1) This paper N/A pCAGGS – PB1 D446Y (catalytically dead), PA E410A (replication-deficient), PA D108A (transcription-deficient) A/Puerto Rico/8/ 1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA I38T (BXAresistant)/NP A/turkey/England/50-92/ 1991 (H5N1) Barclay Lab N/A pPOLI-Firefly luciferase Barclay Lab N/A (Continued on next page) Cell Chemical Biology 33, 1–15.e1–e7, March 19, 2026 e2 .. Human engineered haploid (eHAP; Horizon Discovery) cells and eHAP cells with ANP32A, ANP32B, and ANP32E knocked out by CRISPR-Cas9 31 were maintained in Iscove modified Dulbecco medium (IMDM; Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Labtech), 1% non-essential amino acids (NEAA; Gibco, Life Technologies), and 1% penicillin-streptomycin (pen-strep; Gibco, Life Technologies).
Quantitative RT-PCR:Article Title: Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RNeasy RNA Extraction Mini Kit Qiagen Cat#74106 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit ThermoFisher Scientific Cat#A48310 AgPath-ID One-Step RT-PCR Reagents Life Technologies Cat#4387391 Deposited data Mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al. 2025 56 ) partner repository PXD064673 Cytoscape network NDEx (Pratt et al. 2015 57 ) https://doi.org/10.18119/N9SW4D Experimental models: Cell lines eHAP WT Horizon discovery N/A eHAP ANP32A/B/E-KO Barclay Lab (Sheppard et al. 2023 31 ) N/A .. A549-dox-Cas9 Martin-Sancho Lab (Martin-Sancho et al. 2021 58 ) N/A A549 WT This paper N/A A549 HMGB2-KO This paper N/A A549 RUVBL2-KO This paper N/A A549 RPAP2-KO This paper N/A 293T ATCC N/A MDCK ATCC N/A BCi-NS1.1 Ronald G. Crystal (Walters et al. 2013 59 ) N/A Oligonucleotides qPCR primers; see Table S4 This paper N/A vRNA, cRNA, mRNA, GAPDH RT-qPCR primers; see Table S4 This paper and Swann et al. 2023 11 N/A Primers for validation of CRISPR-Cas9 KO clones; see Table S4 This paper N/A siRNA pools, siNT, siANP32A, siANP32B; see Table S4 siGENOME, Dharmacon N/A sgRNA pools targeting HMGB2, RUVBL2, and RPAP2; see Table S4 Synthego N/A Negative control SpCas9 gRNA #1 Synthego N/A Recombinant DNA pCAGGS-PB1/PB2/PA/NP A/Puerto Rico/ 8/1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/England/195/ 2009 (pH1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/Victoria/3/ 75 (H3N2) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/turkey/ Turkey/05/2005 (H5N1) Barclay Lab N/A pCAGGS-PB1-SmBiT A/Puerto Rico/8/ 1934 (H1N1) This paper N/A pCAGGS – PB1 D446Y (catalytically dead), PA E410A (replication-deficient), PA D108A (transcription-deficient) A/Puerto Rico/8/ 1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA I38T (BXAresistant)/NP A/turkey/England/50-92/ 1991 (H5N1) Barclay Lab N/A pPOLI-Firefly luciferase Barclay Lab N/A (Continued on next page) Cell Chemical Biology 33, 1–15.e1–e7, March 19, 2026 e2 .. Human engineered haploid (eHAP; Horizon Discovery) cells and eHAP cells with ANP32A, ANP32B, and ANP32E knocked out by CRISPR-Cas9 31 were maintained in Iscove modified Dulbecco medium (IMDM; Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Labtech), 1% non-essential amino acids (NEAA; Gibco, Life Technologies), and 1% penicillin-streptomycin (pen-strep; Gibco, Life Technologies).
Biomarker Discovery:Article Title: Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RNeasy RNA Extraction Mini Kit Qiagen Cat#74106 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit ThermoFisher Scientific Cat#A48310 AgPath-ID One-Step RT-PCR Reagents Life Technologies Cat#4387391 Deposited data Mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al. 2025 56 ) partner repository PXD064673 Cytoscape network NDEx (Pratt et al. 2015 57 ) https://doi.org/10.18119/N9SW4D Experimental models: Cell lines eHAP WT Horizon discovery N/A eHAP ANP32A/B/E-KO Barclay Lab (Sheppard et al. 2023 31 ) N/A .. A549-dox-Cas9 Martin-Sancho Lab (Martin-Sancho et al. 2021 58 ) N/A A549 WT This paper N/A A549 HMGB2-KO This paper N/A A549 RUVBL2-KO This paper N/A A549 RPAP2-KO This paper N/A 293T ATCC N/A MDCK ATCC N/A BCi-NS1.1 Ronald G. Crystal (Walters et al. 2013 59 ) N/A Oligonucleotides qPCR primers; see Table S4 This paper N/A vRNA, cRNA, mRNA, GAPDH RT-qPCR primers; see Table S4 This paper and Swann et al. 2023 11 N/A Primers for validation of CRISPR-Cas9 KO clones; see Table S4 This paper N/A siRNA pools, siNT, siANP32A, siANP32B; see Table S4 siGENOME, Dharmacon N/A sgRNA pools targeting HMGB2, RUVBL2, and RPAP2; see Table S4 Synthego N/A Negative control SpCas9 gRNA #1 Synthego N/A Recombinant DNA pCAGGS-PB1/PB2/PA/NP A/Puerto Rico/ 8/1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/England/195/ 2009 (pH1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/Victoria/3/ 75 (H3N2) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/turkey/ Turkey/05/2005 (H5N1) Barclay Lab N/A pCAGGS-PB1-SmBiT A/Puerto Rico/8/ 1934 (H1N1) This paper N/A pCAGGS – PB1 D446Y (catalytically dead), PA E410A (replication-deficient), PA D108A (transcription-deficient) A/Puerto Rico/8/ 1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA I38T (BXAresistant)/NP A/turkey/England/50-92/ 1991 (H5N1) Barclay Lab N/A pPOLI-Firefly luciferase Barclay Lab N/A (Continued on next page) Cell Chemical Biology 33, 1–15.e1–e7, March 19, 2026 e2 .. Human engineered haploid (eHAP; Horizon Discovery) cells and eHAP cells with ANP32A, ANP32B, and ANP32E knocked out by CRISPR-Cas9 31 were maintained in Iscove modified Dulbecco medium (IMDM; Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Labtech), 1% non-essential amino acids (NEAA; Gibco, Life Technologies), and 1% penicillin-streptomycin (pen-strep; Gibco, Life Technologies).
CRISPR:Article Title: Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RNeasy RNA Extraction Mini Kit Qiagen Cat#74106 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit ThermoFisher Scientific Cat#A48310 AgPath-ID One-Step RT-PCR Reagents Life Technologies Cat#4387391 Deposited data Mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al. 2025 56 ) partner repository PXD064673 Cytoscape network NDEx (Pratt et al. 2015 57 ) https://doi.org/10.18119/N9SW4D Experimental models: Cell lines eHAP WT Horizon discovery N/A eHAP ANP32A/B/E-KO Barclay Lab (Sheppard et al. 2023 31 ) N/A .. A549-dox-Cas9 Martin-Sancho Lab (Martin-Sancho et al. 2021 58 ) N/A A549 WT This paper N/A A549 HMGB2-KO This paper N/A A549 RUVBL2-KO This paper N/A A549 RPAP2-KO This paper N/A 293T ATCC N/A MDCK ATCC N/A BCi-NS1.1 Ronald G. Crystal (Walters et al. 2013 59 ) N/A Oligonucleotides qPCR primers; see Table S4 This paper N/A vRNA, cRNA, mRNA, GAPDH RT-qPCR primers; see Table S4 This paper and Swann et al. 2023 11 N/A Primers for validation of CRISPR-Cas9 KO clones; see Table S4 This paper N/A siRNA pools, siNT, siANP32A, siANP32B; see Table S4 siGENOME, Dharmacon N/A sgRNA pools targeting HMGB2, RUVBL2, and RPAP2; see Table S4 Synthego N/A Negative control SpCas9 gRNA #1 Synthego N/A Recombinant DNA pCAGGS-PB1/PB2/PA/NP A/Puerto Rico/ 8/1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/England/195/ 2009 (pH1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/Victoria/3/ 75 (H3N2) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/turkey/ Turkey/05/2005 (H5N1) Barclay Lab N/A pCAGGS-PB1-SmBiT A/Puerto Rico/8/ 1934 (H1N1) This paper N/A pCAGGS – PB1 D446Y (catalytically dead), PA E410A (replication-deficient), PA D108A (transcription-deficient) A/Puerto Rico/8/ 1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA I38T (BXAresistant)/NP A/turkey/England/50-92/ 1991 (H5N1) Barclay Lab N/A pPOLI-Firefly luciferase Barclay Lab N/A (Continued on next page) Cell Chemical Biology 33, 1–15.e1–e7, March 19, 2026 e2 .. Human engineered haploid (eHAP; Horizon Discovery) cells and eHAP cells with ANP32A, ANP32B, and ANP32E knocked out by CRISPR-Cas9 31 were maintained in Iscove modified Dulbecco medium (IMDM; Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Labtech), 1% non-essential amino acids (NEAA; Gibco, Life Technologies), and 1% penicillin-streptomycin (pen-strep; Gibco, Life Technologies).
Clone Assay:Article Title: Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RNeasy RNA Extraction Mini Kit Qiagen Cat#74106 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit ThermoFisher Scientific Cat#A48310 AgPath-ID One-Step RT-PCR Reagents Life Technologies Cat#4387391 Deposited data Mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al. 2025 56 ) partner repository PXD064673 Cytoscape network NDEx (Pratt et al. 2015 57 ) https://doi.org/10.18119/N9SW4D Experimental models: Cell lines eHAP WT Horizon discovery N/A eHAP ANP32A/B/E-KO Barclay Lab (Sheppard et al. 2023 31 ) N/A .. A549-dox-Cas9 Martin-Sancho Lab (Martin-Sancho et al. 2021 58 ) N/A A549 WT This paper N/A A549 HMGB2-KO This paper N/A A549 RUVBL2-KO This paper N/A A549 RPAP2-KO This paper N/A 293T ATCC N/A MDCK ATCC N/A BCi-NS1.1 Ronald G. Crystal (Walters et al. 2013 59 ) N/A Oligonucleotides qPCR primers; see Table S4 This paper N/A vRNA, cRNA, mRNA, GAPDH RT-qPCR primers; see Table S4 This paper and Swann et al. 2023 11 N/A Primers for validation of CRISPR-Cas9 KO clones; see Table S4 This paper N/A siRNA pools, siNT, siANP32A, siANP32B; see Table S4 siGENOME, Dharmacon N/A sgRNA pools targeting HMGB2, RUVBL2, and RPAP2; see Table S4 Synthego N/A Negative control SpCas9 gRNA #1 Synthego N/A Recombinant DNA pCAGGS-PB1/PB2/PA/NP A/Puerto Rico/ 8/1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/England/195/ 2009 (pH1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/Victoria/3/ 75 (H3N2) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/turkey/ Turkey/05/2005 (H5N1) Barclay Lab N/A pCAGGS-PB1-SmBiT A/Puerto Rico/8/ 1934 (H1N1) This paper N/A pCAGGS – PB1 D446Y (catalytically dead), PA E410A (replication-deficient), PA D108A (transcription-deficient) A/Puerto Rico/8/ 1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA I38T (BXAresistant)/NP A/turkey/England/50-92/ 1991 (H5N1) Barclay Lab N/A pPOLI-Firefly luciferase Barclay Lab N/A (Continued on next page) Cell Chemical Biology 33, 1–15.e1–e7, March 19, 2026 e2 .. Human engineered haploid (eHAP; Horizon Discovery) cells and eHAP cells with ANP32A, ANP32B, and ANP32E knocked out by CRISPR-Cas9 31 were maintained in Iscove modified Dulbecco medium (IMDM; Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Labtech), 1% non-essential amino acids (NEAA; Gibco, Life Technologies), and 1% penicillin-streptomycin (pen-strep; Gibco, Life Technologies).
Negative Control:Article Title: Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RNeasy RNA Extraction Mini Kit Qiagen Cat#74106 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit ThermoFisher Scientific Cat#A48310 AgPath-ID One-Step RT-PCR Reagents Life Technologies Cat#4387391 Deposited data Mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al. 2025 56 ) partner repository PXD064673 Cytoscape network NDEx (Pratt et al. 2015 57 ) https://doi.org/10.18119/N9SW4D Experimental models: Cell lines eHAP WT Horizon discovery N/A eHAP ANP32A/B/E-KO Barclay Lab (Sheppard et al. 2023 31 ) N/A .. A549-dox-Cas9 Martin-Sancho Lab (Martin-Sancho et al. 2021 58 ) N/A A549 WT This paper N/A A549 HMGB2-KO This paper N/A A549 RUVBL2-KO This paper N/A A549 RPAP2-KO This paper N/A 293T ATCC N/A MDCK ATCC N/A BCi-NS1.1 Ronald G. Crystal (Walters et al. 2013 59 ) N/A Oligonucleotides qPCR primers; see Table S4 This paper N/A vRNA, cRNA, mRNA, GAPDH RT-qPCR primers; see Table S4 This paper and Swann et al. 2023 11 N/A Primers for validation of CRISPR-Cas9 KO clones; see Table S4 This paper N/A siRNA pools, siNT, siANP32A, siANP32B; see Table S4 siGENOME, Dharmacon N/A sgRNA pools targeting HMGB2, RUVBL2, and RPAP2; see Table S4 Synthego N/A Negative control SpCas9 gRNA #1 Synthego N/A Recombinant DNA pCAGGS-PB1/PB2/PA/NP A/Puerto Rico/ 8/1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/England/195/ 2009 (pH1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/Victoria/3/ 75 (H3N2) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/turkey/ Turkey/05/2005 (H5N1) Barclay Lab N/A pCAGGS-PB1-SmBiT A/Puerto Rico/8/ 1934 (H1N1) This paper N/A pCAGGS – PB1 D446Y (catalytically dead), PA E410A (replication-deficient), PA D108A (transcription-deficient) A/Puerto Rico/8/ 1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA I38T (BXAresistant)/NP A/turkey/England/50-92/ 1991 (H5N1) Barclay Lab N/A pPOLI-Firefly luciferase Barclay Lab N/A (Continued on next page) Cell Chemical Biology 33, 1–15.e1–e7, March 19, 2026 e2 .. Human engineered haploid (eHAP; Horizon Discovery) cells and eHAP cells with ANP32A, ANP32B, and ANP32E knocked out by CRISPR-Cas9 31 were maintained in Iscove modified Dulbecco medium (IMDM; Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Labtech), 1% non-essential amino acids (NEAA; Gibco, Life Technologies), and 1% penicillin-streptomycin (pen-strep; Gibco, Life Technologies).
Recombinant:Article Title: Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RNeasy RNA Extraction Mini Kit Qiagen Cat#74106 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit ThermoFisher Scientific Cat#A48310 AgPath-ID One-Step RT-PCR Reagents Life Technologies Cat#4387391 Deposited data Mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al. 2025 56 ) partner repository PXD064673 Cytoscape network NDEx (Pratt et al. 2015 57 ) https://doi.org/10.18119/N9SW4D Experimental models: Cell lines eHAP WT Horizon discovery N/A eHAP ANP32A/B/E-KO Barclay Lab (Sheppard et al. 2023 31 ) N/A .. A549-dox-Cas9 Martin-Sancho Lab (Martin-Sancho et al. 2021 58 ) N/A A549 WT This paper N/A A549 HMGB2-KO This paper N/A A549 RUVBL2-KO This paper N/A A549 RPAP2-KO This paper N/A 293T ATCC N/A MDCK ATCC N/A BCi-NS1.1 Ronald G. Crystal (Walters et al. 2013 59 ) N/A Oligonucleotides qPCR primers; see Table S4 This paper N/A vRNA, cRNA, mRNA, GAPDH RT-qPCR primers; see Table S4 This paper and Swann et al. 2023 11 N/A Primers for validation of CRISPR-Cas9 KO clones; see Table S4 This paper N/A siRNA pools, siNT, siANP32A, siANP32B; see Table S4 siGENOME, Dharmacon N/A sgRNA pools targeting HMGB2, RUVBL2, and RPAP2; see Table S4 Synthego N/A Negative control SpCas9 gRNA #1 Synthego N/A Recombinant DNA pCAGGS-PB1/PB2/PA/NP A/Puerto Rico/ 8/1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/England/195/ 2009 (pH1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/Victoria/3/ 75 (H3N2) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/turkey/ Turkey/05/2005 (H5N1) Barclay Lab N/A pCAGGS-PB1-SmBiT A/Puerto Rico/8/ 1934 (H1N1) This paper N/A pCAGGS – PB1 D446Y (catalytically dead), PA E410A (replication-deficient), PA D108A (transcription-deficient) A/Puerto Rico/8/ 1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA I38T (BXAresistant)/NP A/turkey/England/50-92/ 1991 (H5N1) Barclay Lab N/A pPOLI-Firefly luciferase Barclay Lab N/A (Continued on next page) Cell Chemical Biology 33, 1–15.e1–e7, March 19, 2026 e2 .. Human engineered haploid (eHAP; Horizon Discovery) cells and eHAP cells with ANP32A, ANP32B, and ANP32E knocked out by CRISPR-Cas9 31 were maintained in Iscove modified Dulbecco medium (IMDM; Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Labtech), 1% non-essential amino acids (NEAA; Gibco, Life Technologies), and 1% penicillin-streptomycin (pen-strep; Gibco, Life Technologies).
Luciferase:Article Title: Influenza A virus polymerase co-opts distinct sets of host proteins for RNA transcription or replication.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER RNeasy RNA Extraction Mini Kit Qiagen Cat#74106 MagMAX Viral/Pathogen Nucleic Acid Isolation Kit ThermoFisher Scientific Cat#A48310 AgPath-ID One-Step RT-PCR Reagents Life Technologies Cat#4387391 Deposited data Mass spectrometry proteomics data ProteomeXchange Consortium via the PRIDE (Perez-Riverol et al. 2025 56 ) partner repository PXD064673 Cytoscape network NDEx (Pratt et al. 2015 57 ) https://doi.org/10.18119/N9SW4D Experimental models: Cell lines eHAP WT Horizon discovery N/A eHAP ANP32A/B/E-KO Barclay Lab (Sheppard et al. 2023 31 ) N/A .. A549-dox-Cas9 Martin-Sancho Lab (Martin-Sancho et al. 2021 58 ) N/A A549 WT This paper N/A A549 HMGB2-KO This paper N/A A549 RUVBL2-KO This paper N/A A549 RPAP2-KO This paper N/A 293T ATCC N/A MDCK ATCC N/A BCi-NS1.1 Ronald G. Crystal (Walters et al. 2013 59 ) N/A Oligonucleotides qPCR primers; see Table S4 This paper N/A vRNA, cRNA, mRNA, GAPDH RT-qPCR primers; see Table S4 This paper and Swann et al. 2023 11 N/A Primers for validation of CRISPR-Cas9 KO clones; see Table S4 This paper N/A siRNA pools, siNT, siANP32A, siANP32B; see Table S4 siGENOME, Dharmacon N/A sgRNA pools targeting HMGB2, RUVBL2, and RPAP2; see Table S4 Synthego N/A Negative control SpCas9 gRNA #1 Synthego N/A Recombinant DNA pCAGGS-PB1/PB2/PA/NP A/Puerto Rico/ 8/1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/England/195/ 2009 (pH1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/Victoria/3/ 75 (H3N2) Barclay Lab N/A pCAGGS-PB1/PB2/PA/NP A/turkey/ Turkey/05/2005 (H5N1) Barclay Lab N/A pCAGGS-PB1-SmBiT A/Puerto Rico/8/ 1934 (H1N1) This paper N/A pCAGGS – PB1 D446Y (catalytically dead), PA E410A (replication-deficient), PA D108A (transcription-deficient) A/Puerto Rico/8/ 1934 (H1N1) Barclay Lab N/A pCAGGS-PB1/PB2/PA I38T (BXAresistant)/NP A/turkey/England/50-92/ 1991 (H5N1) Barclay Lab N/A pPOLI-Firefly luciferase Barclay Lab N/A (Continued on next page) Cell Chemical Biology 33, 1–15.e1–e7, March 19, 2026 e2 .. Human engineered haploid (eHAP; Horizon Discovery) cells and eHAP cells with ANP32A, ANP32B, and ANP32E knocked out by CRISPR-Cas9 31 were maintained in Iscove modified Dulbecco medium (IMDM; Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS; Labtech), 1% non-essential amino acids (NEAA; Gibco, Life Technologies), and 1% penicillin-streptomycin (pen-strep; Gibco, Life Technologies).
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